Demonstration experimental system for water treatment based on singlet oxygen micro-nanobubbles

Project code:
PN-III-P2-2.1-PED-2019-4771
Project number:
PED 456/2020
Funding bodies:
MCI, UEFISCDI
Programme:
Demonstration experimental project (PED)
Coordinator:
National Institute for Laser, Plasma and Radiation Physics (INFLPR)
Project Leader:
Dr. Staicu Angela
Start date:
10.11.2020
End date:
09.11.2022
Summary

The project main objective is to develop and test a demonstration experimental system for water decontamination based on the action of micro and nano bubbles (micro-nanobubbles) containing reactive oxygen species such as singlet oxygen. The micro-nanobubbles include singlet oxygen and are generated by a photoactivated porous membrane containing photosensitizers functionalised carbon nanotubes and TiO2 nanoparticles.

Expected results

*achievement and characterization (spectroscopic, photophysical, optical, microfluidic) of the membrane containing the photoactive species (TiO2, PS), * the development of micro-nanobubbles generator system, find the proper functional parameters; *the development of irradiation system, establish its proper configuration and parameters; *assembling the laboratory experimental set-up, adjusting an optimization of its functional parameters, *test and validation of the system, asses the capacity for water decontamination of organic pollutants and disinfection efficiency by biological assay.

Project Team
Project Leader:
Dr. Angela Staicu, CS I
Team Members:
Dr. Ionut Relu Andrei, CS III
Dr. Adriana Smarandache, CS III
Dr. Mihai Boni, CS III
Dr. Mihai Boni, CS III
Dr. Andra Dinache, CS III
Dr. Tatiana Tozar, CS III
Dr. Iuliana Urzica, CS III
Dr. Ana-Maria Udrea, CS
Simona Ioana Nistorescu, ACS
Dr. Mihail-Lucian Pascu, CS I
Outreach of the project

Stage Reports

Nov-Dec 2020

Stage 1: Establish experimental set-up components functional parameters (I)

Summary Stage Report I

Activity 1.1: Up-date of the concept model considering the latest literature reports and purchase of components.

Objectives

Up-date about the concept model within project specific detailed activities considering the latest literature reports.

Selection and purchase of the experimental set-up proper components (I).

Selection of the proper compounds.

Jan-Dec 2021

Stage 2: Establish experimental set-up components functional parameters (II). Development of the nanocomplexes and their photophysical and spectral characterization. Development of system components, adjust their parameters and test their functionality.

Summary Stage Report II

Activity 2.1: Synthesis of the nano-compounds and efficiency measurements. Achievement and characterization of the membrane. Development and optimization of the micro-nanobubbles generation system.

Objectives

Selection and purchase of the experimental set-up proper components (II). Test subassemblies.

Synthesis of the nano-complexes. Spectral characterization (FTIR, UV-VIS absorbance, singlet oxygen generation). Efficiency in photodegradation of organic pollutants.

Development of micro-nanobubbles generation system – establish functional parameters, optimize the bubble dimensions measured by a fast camera.

Achievement of PS–CNT-TiO2 membrane – spectral, microscopic characterization – irradiation parameters – singlet oxygen generation efficiency. Measurements of fluidic properties (contact angles).

Deliverables

*Micro-nanobubbles generation system, characteristics, and optimized functional parameters; *PS – CNT-TiO2 membrane, characteristics and irradiation parameters; *Dissemination of results by publishing 1 papers in ISI quoted journal and communications at one international conference; *weekly meetings, intermediate reports, final phase report.

Disemination

Articles

Udrea, A.M.; Dinache, A.; Pagès, J.M.; Pirvulescu, R.A., "Quinazoline Derivatives Designed as Efflux Pump Inhibitors: Molecular Modeling and Spectroscopic Studies", Molecules , 26, 2374 (2021)

Andrei, I.R.; Boni, M.; Staicu, A.; Pascu, M.L., "Scattering resonances observed in the lasing emission spectrum of large dye-doped droplets", Optics & Laser Technology, Vol. 140, 107088, (2021)

Avram, S.;Udrea, A.M.; Nuta, D.C.; Limban, C.; Balea, A.C.; Caproiu, M.T.; Dumitrascu, F.; Buiu, C.; Bordei, A.T., "Synthesis and Bioinformatic Characterization of New Schiff Bases with Possible Applicability in Brain Disorders", Molecules, 26, 4160 (2021)

Avram, S.; Stan, M.S.; Udrea, A.M.; Buiu, C.; Boboc, A.A.; Mernea, M., "3D-ALMOND-QSAR Models to Predict the Antidepressant Effect of Some Natural Compounds", Pharmaceutics, 13, 1449 (2021)

Udrea, A.M.; Gradisteanu Pircalabioru, G.; Boboc, A.A.; Mares, C.; Dinache, A.; Mernea, M.; Avram, S., "Advanced Bioinformatics Tools in the Pharmacokinetic Profiles of Natural and Synthetic Compounds with Anti-Diabetic Activity", Biomolecules, 11, 1692 (2021)

Nistorescu, S.; Udrea, A.M.; Badea, M.A.; Lungu, I.; Boni, M.; Tozar, T.; Dumitrache, F.; Maraloiu, V.A.; Popescu, R.G.; Fleaca, C.; Andronescu, E.; Dinischiotu, A.; Staicu, A.; Balas, M. Low Blue Dose Photodynamic Therapy with Porphyrin-Iron Oxide Nanoparticles Complexes: In Vitro Study on Human Melanoma Cells. Pharmaceutics 2021, Special Issue "Novel Anticancer Strategies (Volume II)"- Accepted Date: 3 December 2021.

Conferences

Dinache, A.; Tozar, T.; Smarandache, A.; Andrei, I.R.; Boni, M.; Staicu, A.; Pascu, M.L.; Laser Assisted Generation of Medicine Emulsions; PortASAP Meeting Rome 2021, virtual conference, Italy, 10-11 February 2021

Dinache A., Tozar T., Smarandache A., Andrei I.R., Boni M., Pascu M.L., Staicu A.; The effect of laser radiation on drug emulsions; 9th International conference on radiation in various fields of research, virtual conference, Montenegro, 14-18 June 2021

Udrea, A.M.; Stroescu, S.; Boni, M.; Pascu, ML; Staicu, A. “Mechanism of action prediction of TPPS4 photosensitiser in melanoma treatment using molecular docking”; Bucharest University Faculty of Physics 2021 Meeting, 18-06-2021 - Bucharest, Romania, Oral presentation

Jan-Nov 2022

Stage 3: Development of the demonstration experimental system for water decontamination. Validation of the demonstration laboratory model.

Summary Stage Report III

Activity 3.1: Development, testing and validation of the demonstration laboratory model for water decontamination.

Objectives

Build-up and functionality tests of the demonstration laboratory model for water decontamination.

Developing biological assay for testing the antibacterial photodynamic efficiency of the developed system.

Final tests and validation of the demonstration laboratory system.

Elaboration of final technical and scientific report for the demonstration system.

Deliverables

*Demonstration laboratory system for water decontamination, optimized functional parameters; *Developing biological assay for testing the antibacterial photodynamic efficiency of the developed system; *Dissemination of results by publishing 2 papers in ISI quoted journals and communications at two international conferences; *weekly meetings, intermediate reports, final phase report.

Disemination

Articles

Tozar, T.; Nistorescu, S.I.; Boni, M.; Gradisteanu Pircalabioru, G.; Negut, I.; Staicu, A.; "Pulsed laser photo-crosslinking of gelatin methacryloyl hydrogels for the controlled delivery of chlorpromazine to combat antimicrobial resistance", Pharmaceutics 14, 2121 (2022)

Udrea, A.M.; Dinache, A.; Staicu, A.; Avram, S.; "Target Prediction of 5,10,15,20-Tetrakis(4′-Sulfonatophenyl)-Porphyrin Using Molecular Docking" Pharmaceutics 14(11), 2390 (2022)

Dinache, A.; Nistorescu, S.I.; Tozar, T.; Smarandache, A.; Boni, M.; Staicu, A.; "Spectroscopic Investigations of Porphyrin-TiO2 Nanoparticles Complexes" Molecules (2022) under review

Conferences

Udrea, A.M.; Staicu, A.; Mares, C.; Avram; S.; "Predictions of the TPPS photosensitizer pharmacodynamics as a cancer treatment using molecular docking" ICLPR-ST 2022, 7-10 June 2022, Bucharest, Romania

Udrea, A.M.; Nistorescu, S.I.; Pascu, M.L.; Romanitan, M.O.; Avram, S.; "In silico study of laser-irradiated PZ in interaction with the SARS-Cov-2 Mpro" ICLPR-ST 2022, 7-10 June 2022, Bucharest, Romania.

Boni, M.; Nistorescu, S.I.; Tozar, T.; Dinache, A.; Stanciu, G.; Udrea, A.M.; Simon, A.; Staicu, A.; "System for Water Treatment Based on Singlet OxygenMicro-nanobubbles", ICLPR-ST 2022, 7-10, June 2022, Bucharest, Romania."

Dinache, A.; Nistorescu, S.I.; Smarandache, A.; Tozar, T.; Boni, M.; Udrea, A.M.; Staicu, A.; "Photophysical properties of porphyrins loaded TiO2 nanoparticles" ICLPR-ST 2022, 7-10 June 2022, Bucharest

Andrei, I.R.; Stancalie, A.; Boni, M.; Pascu, M.L.; Bulinski, M.; Micro-droplet temperature characterisation using a fibre Bragg grating, ICLPR-ST 2022, June 7-10, 2022, Bucharest, Romania.

SMARANDACHE, A.; Möller, R.; PASCU, M.L.; "Stability investigation of gamma-ray irradiated antibiotics", RAD 2022 Conference (Spring Edition), 13-17 June 2022, Herceg-Novi, Montenegro

Udrea, A.M.; Staicu, A.; Avram; S.; "The interactions between photosensitizer and cancer therapeutical targets—a computational approach" RAD 10 th Jubille, Spring Edition, 13-17 June 2022, Herceg Novi, Montenegro.

Dinache, A.; Nistorescu, S.I.; Smarandache, A.; Tozar, T.; Boni, M.; Staicu, A.; "Spectral Characterization of TiO2 Nanoparticles Functionalized with Porphyrins", 20th International Balkan Workshop on Applied Physics and Materials Science, 12-15 July 2022, Constanta, Romania