Current Funded Projects
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Generation and identification of antimicrobial species from medicines exposed to laser radiation in view of fighting multiple drug resistance acquired by bacteria (ANTLAS)
One of the most important current drawbacks that have to be addressed in fighting infections with multiple drug resistant bacteria is the ineffectiveness of current antibiotics to destroy them and the lack of new antibiotic molecules and of new treatment schemes. At the same time, a fast and cheap approach for drug development is needed such as exposure of existing drugs to laser beams. Then, identification of new drugs must be made, which implies in most cases HPLC systems. A better solution is the high-performance thin layer chromatography (HPTLC), an offline method that is superior to other analytical techniques in terms of total costs and time for analysis. Development of a HPTLC single track scanner to identify new photoproducts is proposed in this project, for which low-intensity monochromatic light generated by picosecond lasers is used. The signal obtained from the HPTLC plates is analysed with a spectrograph equipped with a CCD camera for fluorescence spectra monitoring and a photomultiplier coupled to an oscilloscope for fluorescence lifetime evaluation. The overall objective of the proposal is to photo-generate antimicrobial species by exposing current drugs to UV laser beam and to characterize and identify the new species by performing qualitative and quantitative analysis using an improved HPTLC densitometry system. The results concerning the new species will be correlated with mass spectrometry measurements performed by LC-TOF/MS and the molecular structures of the antimicrobial compounds will be validated with Gaussian09 software.